High-resolution imaging with field-emission SEM: the need for downstream plasma cleaning in biological applications

For high-resolution visualization and image capturing of biological samples, the cleanliness of the high-vacuum imaging environment, including both instrument and sample surface, have become important aspects to ensure electron beam stability and high signal-to-noise ratios (SNR). Regular downstream plasma cleaning of both SEM chamber and specimen is required to ensure an uncontaminated instrument that can deliver accurate, reliable and repeatable secondary and backscattered electron data. Development of new imaging technologies and sample requirements may provide novel challenges to instrument and electron microscopist. Control of a clean imaging environment through regular plasma cleaning, prevents contamination build-up throughout the SEM, and results in improved beam stability and SNR for highresolution biological imaging.